The Human Genome Illustrates Both Continuity And Change

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The Human Genome Illustrates Both Continuity And Change

. a. Describe the essential features of TWO of the procedures/techniques below. For each of the procedures/techniques you describe, explain how its application contributes to understanding genetics. -The use of a bacterial plasmid to clone and sequence a human gene -Polymerase chain reaction (PCR) -Restriction fragment length polymorphism (RFLP) analysis
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-The use of a bacterial plasmid to clone and sequence a human gene Description: use restriction enzymes to cut out a piece of the plasmid cut human sequence with the corresponding restriction enzyme insert the new DNA from the plasmid into the human cell use gel electrophoresis to separate the fragments and read the sequence Contribution: used to create transgenic organisms used to create human proteins highly helpful in the development of treatment for genetic diseases can produce insulin -Polymerase chain reaction (PCR) Description: uses heat to separate DNA strands add primers add polymerase and/or nucleotides allows for the amplification of a piece of DNA Contribution: used to study a particular segment of DNA can be used in forensic studies or studies in which a scientist only has a small portion of DNA and needs more to be able to fully analyze it

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