Pulse-Chase Experiments And Protein Location

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Pulse-Chase Experiments And Protein Location

Scientists can track the movement of proteins through the endomembrane system using an approach known as a pulse-chase experiment. This experiment involves the "pulse" phase: Cells are exposed to a high concentration of a radioactively labeled amino acid for a short period to tag proteins that are being synthesized. the "chase" phase: Any unincorporated radioactively labeled amino acids are washed away and large amounts of the same, but unlabeled, amino acid are added. Only those proteins synthesized during the brief pulse phase are radioactively tagged. These tagged proteins can be tracked through the chase period to determine their location in the cell. The data below were obtained from a pulse-chase experiment in which cells were examined at different times during the chase period. The numbers represent the radioactivity (measured in counts per minute) recorded at each of the indicated sites. The higher the number, the greater the radioactivity. Based on these data, what is the most likely function of the cells in this experiment? secretion phagocytosis detoxification muscle contraction
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The cells in this experiment were macrophages. These immune system cells have many lysosomes for the destruction of bacteria and other invaders brought into the cell via phagocytosis. The enzymes (hydrolases) that carry out this catabolic activity are synthesized in the endoplasmic reticulum, modified in the Golgi, and transported to the lysosomes.
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