Which Of The Following Is Not Correct When Considering The Initiation Of Transcription In E. Coli?

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Which Of The Following Is Not Correct When Considering The Initiation Of Transcription In E. Coli?

1.The phosphodiester bonds that are formed are not stable until about 10 nucleotides have been incorporated. 2.Only the -10 region of the promoter is required. 3.The RNA polymerase contacts about 50 bp of DNA. 4.The _ subunit of RNA polymerase makes strong contacts with the -10 region of promoters. 5.DNA melting near the transcription start site is essential for promoter recognition.
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