How Many Bands Will Be Visible On The Gel Assuming All Unprotected Dna Was Cleaved?

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How Many Bands Will Be Visible On The Gel Assuming All Unprotected Dna Was Cleaved?

es in the manner typical of eukaryotic genomes, with 145 bp encircling each nucleosome and approximately 55 bp in linker DNA. When C. elegans chromatin is carefully isolated, stripped of nonhistone proteins, and placed in an appropriate buffer, the chromatin decondenses to the 10-nm fiber structure. Suppose researchers mix a sample of 10-nm-fiber chromatin with a large amount of the enzyme DNase I that randomly cleaves DNA in regions that are not protected by bound protein. Next, they remove the nucleosomes, separate the DNA fragments by gel electrophoresis, and stain the fragments by ethidium bromide. (How many bands will be visible on the gel assuming all unprotected DNA was cleaved?)
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