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Why Is Primase Required For Replication?

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Primase is a DNA-dependent RNA polymerase. Primase synthesizes the short RNA molecules, or primers, that have a free 3'-OH to which DNA polymerase can attach deoxyribonucleotides in replication initiation. The DNA polymerases require a free 3'-OH to which they add nucleotides, and therefore they cannot themselves initiate replication. Primase does not have this requirement.

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