Why Is The Dna Sample To Be Separated By Gel Electrophoresis Always Loaded At The Cathode

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Why Is The Dna Sample To Be Separated By Gel Electrophoresis Always Loaded At The Cathode

or negative end of the power source?
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The gel acts as a molecular sieve: because nucleic acid molecules carry negative charges on their phosphate groups, they all travel toward the positive pole in an electric field. As they move, the thicket of agarose fibers impedes longer molecules more than it does shorter ones, separating them by length. Thus, a mixture of linear DNA molecules is separated into bands, each consisting of many thousands of DNA molecules of the same length.
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